Sofia Zoo has a volunteer programme and an established Regulation for Volunteer Activities. Volunteers are accepted among adults over 18 years of age who are motivated and have a genuine interest in animals and nature. Prospective volunteers first attend an interview with a staff member from the Ecological Scientific and Educational Centre, which manages the Volunteer Programme. After familiarising themselves with the Regulation, they complete an Application Form for Volunteer Participation as well as a Questionnaire. Once approved for the programme, they may begin their volunteer activities at the zoo. Accepted volunteers are assigned only to activities that correspond to their physical abilities, education, qualifications, and interests.

Volunteers are not assigned tasks involving direct care of the zoo animals. Exceptions are made for animals housed at the Ecological Centre or in other cases at the discretion of the sector supervisors and the Zoo Director.